Merseyrail are seeking an injunction to halt an industrial strike by the Rail, Maritime and Transport workers' union which could bring the network down.
The RMT, which has been central to the prolonged period of strikes on the Southern Rail network, has planned a 24-hour strike over an issue which has plagued Southern Rail for the last year - the role of conducters and driver-only trains (DOO).
The strike is planned for March 13, and now the company has leapt into action to try to prevent the disruption the walkout will cause.
They released a statement confirming their move, saying the RMT was arguing with the wrong people.
It read: "This [the dispute] is in relation to on-board staffing arrangements on the new fleet of trains that Merseytravel will introduce on to the network in 2020.
"Merseyrail argued to the High Court that decisions about the designs and operations of trains under DOO principles were taken by council leaders and councillors in December 2016 and Merseyrail had no choice other than to implement these plans.
"As such, the RMT's dispute is with Merseytravel and not Merseyrail."
The case is set to be heard in London's High Court later on Thursday.