Jacob Rees-Mogg has criticised the "eccentric, angry, frustrated rump who love the EU", as a petition against the suspension of Parliament surpasses one million signatures.
But the House of Commons leader dismissed the number of signatories, saying he will only pay attention when it is "17.5 million and tops the number who voted to leave", branding devoted pro-EU campaigners as "friends of every country but their own".
The 'Do not prorogue Parliament' petition snowballed after Boris Johnson confirmed his plans to suspend Parliament until October 14 yesterday, after approval from the Queen.
Her approval drew criticism from some, who claimed the Royal had been forced into the Brexit row by the Prime Minister.
"People who are saying it has involved the Queen in politics are irresponsible and ought to go back to school to study the constitution," Mr Rees-Mogg told talkRADIO's Julia Hartley-Brewer.
"Her Majesty had no discretion in this whatsoever, there was no precedent of the Queen refusing a new Queen’s speech."
He continued: "It’s standard to have a Queen’s speech every year, the oddity this time is we’ve gone over two years without having a Queen’s speech and we need a new session of Parliament so we can get on with the domestic agenda, get on with reforms to the health service, dealing with knife crime."
Mr Johnson has been accused of proroguing Parliament in an effort to push through a no-deal Brexit, but Mr Rees-Mogg insisted only a "very small number of sitting days will be lost".
"People seem to be imitating rage in a furious fashion," he added.
"We will leave the EU on October 31, thanks be to God."