New polling figures from YouGov suggest support for independence in Scotland has fallen to its lowest level since the 2014 referendum.
The figures show support for independence at just 44%, one percent lower than it was in the plebiscite two years ago.
The survey also suggests that less than a third of Scottish people want the Scottish Government to push for secession over the next two years.
Support for the SNP, the main pro-independence party in Scotland, surged in the wake of the referendum, culminating in the party picking up 56 of Scotland's 59 seats in the 2015 General Election.
SNP leader Nicola Sturgeon has also made several high-profile speeches in the wake of the UK's vote to leave the European Union this year, highlighting the fact that a majority of Scots voted to remain in the EU and the Brexit vote will create a democratic deficit in Scotland.
However critics have suggested the fall in the global price of oil demonstrates the flaws in the case for a Scottish breakaway, given campaigners have suggested North Sea oil revenues would be pivotal to the economy of an independent Scotland.
A spokesman for the Scottish Conservatives was quoted by The Scotsman : “The standout finding from this poll is the increasing opposition to another referendum on independence, and the drop in support for independence itself now lower than at anytime since the 2014 referendum.
“On the day the SNP finish their so-called listening exercise on independence, it is now time they finally paid attention to what Scotland it saying - no to a second referendum and no to more division, more uncertainty and more rancour.”
talkRADIO presenter George Galloway poured scorn on the SNP's independence push in his latest weekly show, suggesting the UK's Brexit vote was not sufficient grounds for a second referendum and Scots must respect the will of the British majority.