Theresa May has been reportedly moving to block any plans for a second Scottish independence referendum until after the UK leaves the European Union.
On Monday, before the bill to trigger Brexit was ratified by the House of Lords, SNP leader Nicola Sturgeon gave a keynote speech on Scottish independence.
Scotland's First Minister publicly said she will seek the approval of Holyrood to hold a vote between autumn of 2018 and spring of 2019.
In response, the Prime Minister, who has previously accused the Scottish National Party of having "tunnel vision", reiterated her claim against the First Minister in an interview with the BBC.
Mrs May accused Mrs Sturgeon of "playing politics with the future of our country" to create "more uncertainly and division" at a time when polls suggest Scotland doesn't want a second vote.
In response to this, the SNP warned the Prime Minister not to get involved.
The party's deputy leader, Angus Robertson, told the BBC: "I just can't see how the Government will say to a democratically elected Scottish Government elected on a mandate to hold a referendum they aren't going to allow a vote."