Donald Trump has demanded Twitter allow “banned Conservative voices” back onto the platform “without restriction”.
In the tweet he wrote: "It’s called Freedom of Speech, remember. You are making a Giant Mistake.”
It’s not the first time the US president has railed against Twitter for blocking its users.
Last month he suggested the Democrats could be partly to blame for the bans.
On May 4 he wrote: “How can it be possible that James Woods (and many others), a strong but responsible Conservative Voice, is banned from Twitter?”
“Social Media & Fake News Media, together with their partner, the Democrat Party, have no idea the problems they are causing for themselves. VERY UNFAIR!”
Conservative actor James Woods was locked out of his Twitter account in April for abusive behaviour.
The tweet that triggered the ban read: “If you try to kill the King, you better not miss. #HangThemAll”
Another high-profile US conservative to be banned is conspiracy theorist Alex Jones.
The controversial radio host was permanently blocked in September last year.
He is best known for spreading a conspiracy theory that the Sandy Hook school shooting was faked.
A Twitter spokesperson said: “We enforce the Twitter Rules impartially for all users, regardless of their background or political affiliation.”
Mr Trump has faced criticism in the past for blocking people from viewing his own Twitter account
In May last year a New York judge ruled Donald Trump cannot block people on Twitter, as it violated their first amendment rights to participate in a “public forum”.
Judge Naomi Reice Buchwald said the @realDonaldTrump account was a “presidential account as opposed to a personal account”.
She recommended the president ignore the comments that upset him.